BPCPPDYSOE21 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 3: Focus Physics of Stem Cells
BP 3.1: Vortrag
Montag, 22. März 2021, 09:00–09:20, BPc
How Tissue Microenvironment Impacts Pluripotent Cell Differentiation — •Allyson Quinn Ryan1,2, Diana Alves-Afonso1, Jacqueline M. Tabler1, and Carl D. Modes1,2 — 1Max Planck Institute for Molecular Cell Biology and Genetics — 2Center for Systems Biology Dresden
The importance of stem cell population maintenance throughout both development and adulthood has been evident for several decades. Classically, how these populations are regulated is investigated through genetic and cell biological studies. However, work in recent years has shown forces exerted by and through tissue microenvironments to be of equal importance as molecular and transcriptional profiles to cell potency and identity. Here we show that collagen organization and tissue stiffness of the midline suture, a stem cell like niche in the cranial mesenchyme, is distinct from that of adjacent tissues. Surprisingly, Lamin A/C nuclear envelope expression is higher in suture than bone, despite the soft nature of the tissue. When collagen crosslinking is perturbed, Lamin A/C localization patterns, nuclear morphology and neighbor relationships within the suture are significantly altered. These results point towards a framework of noncellular tissue entities and collective organization influencing the maintenance of potency in developmental tissues.