BPCPPDYSOE21 – scientific programme
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BP: Fachverband Biologische Physik
BP 7: Cell Mechanics III
BP 7.2: Talk
Monday, March 22, 2021, 14:20–14:40, BPa
Tracking Electrostatically Driven Membrane Transfer between Lipid Vesicles and a Supported Lipid Bilayer on a QCM — •Justus Bednár1,2, Anastasia Svetlova1,2, Vanessa Maybeck1, and Andreas Offenhäusser1 — 1Forschungszentrum Jülich, Institute of Biological Information Processing: Bioelectronics (IBI-3) — 2Fakultät für Mathematik, Informatik und Naturwissenschaften RWTH Aachen
Lipid bilayer systems are used widely in medicine and biotechnology. Supported lipid bilayers (SLBs) for example, can be employed as a biomimetic platform for cell cultures or can be studied as a model system of the cell membrane itself. If SLB and lipid vesicles have opposite surface charges, their electrostatic interaction can be used to modify the lipid composition of the SLB. Studying the underlying process, the quartz crystal microbalance (QCM) stands out for its ability to precisely monitor the acoustic response of a macroscopic SLB and coupled objects with a sub-second time resolution. Unfortunately, standard models that relate the QCM signal response to physical properties of the sample do not apply in this case.
Here, a viscoelastic model for an ensemble of lipid vesicles, coupled to an SLB, is presented. Experimental results demonstrate the capability of this model to estimate relative concentrations of extracellular vesicles (EVs) in bulk solution. Furthermore, throughout numerous experiments of electrostatically driven membrane transfer between lipid vesicles and an SLB, a non-trivial time-dependence of vesicle-adsorption is observed.