BPCPPDYSOE21 – wissenschaftliches Programm

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 16: Charged Soft Matter - organized by Joachim Dzubiella (Albert Ludwigs University Freiburg, Freiburg)

CPP 16.4: Hauptvortrag

Mittwoch, 24. März 2021, 11:00–11:40, CPPa

Interaction of polyelectrolytes with proteins — •Matthias Ballauff — Institut für Chemie und Biochemie, Freie Universitaet Berlin

Highly charged natural polyelectrolytes as e.g. DNA or heparin play a central role in many biochemical processes and their interaction with proteins is of central importance as shown in a recent review [1]. In this lecture I shall review our studies of the interaction of well-defined synthetic polyelectrolytes with proteins that have been done mainly by calorimetry. There are two main factors that lead to the formation of a complex between a protein and a polyelectrolyte: i) The release of counterions condensed onto the highly charged polyelectrolyte, and ii) The release or uptake of water during complex formation. Systematic studies of the binding constant K_b as the function of temperature and salt concentration allow us to determine the contributions of both effects to the measured free energy of binding. This can be demonstrated by analyzing the binding of model proteins as lysozyme to well-characterized polyelectrolytes as e.g. heparin. The improved understanding of this complex formation may have direct medical implications as e.g. for virus binding to cell surfaces.

[1] K. Achazi, et al., Understanding the Interaction of Polyelectrolyte Architectures with Proteins and Biosystems, Angew. Chem. Int. Ed. 2020, 59, 2-25