BPCPPDYSOE21 – scientific programme

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 16: Charged Soft Matter - organized by Joachim Dzubiella (Albert Ludwigs University Freiburg, Freiburg)

CPP 16.7: Talk

Wednesday, March 24, 2021, 12:20–12:40, CPPa

The effects of ethanol and salt on the phase behavior and interactions of aqueous protein solutions — Rajeevann Uthayakumar, •Florian Platten, and Stefan U. Egelhaaf — Condensed Matter Physics Laboratory, Heinrich Heine University, Düsseldorf, Germany

The addition of salts or organic solvents to aqueous protein solutions alters their optical and dielectric properties and the interactions between protein molecules in these mixtures change accordingly. Here, the effects of NaCl and ethanol on the phase behavior and interactions of protein solutions are studied in terms of the metastable liquid-liquid phase separation and second virial coefficient B₂ of lysozyme solutions. The cloud-point temperatures are reduced and raised by the addition of ethanol and salt, respectively. The extended law of corresponding states allows to interpret these trends as changes of B₂. Remarkably, the dependence of B₂ on both salt and ethanol content is quantitatively modelled by the DLVO theory.