SKM 2021 – scientific programme
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BP: Fachverband Biologische Physik
BP 3: Protein Structure and Dynamics
BP 3.2: Talk
Thursday, September 30, 2021, 10:30–10:45, H1
Size-dependent deviations from the colloidal prediction: about the diffusion of proteins in a cellular environment — •Christian Beck1,2, Felix Roosen-Runge3, Tilo Seydel2, and Frank Schreiber1 — 1Institut für Angewandte Physik, Universität Tübingen, 72076 Tübingen, Germany — 2Institut Laue-Langevin, 38042 Grenoble Cedex 9, France — 3Department of Biomedical Science, Malmö University, Sweden
Diffusive properties are of fundamental importance for biological processes. For their quantitative understanding, the short-time diffusive properties are of huge interest. Previous studies investigated the volume fraction dependence of short-time diffusive properties for different pure proteins solutions [1] and recently in controlled poly-disperse cell-like environments [2]. In cooperation with the ILL life science group, we now investigated the diffusive properties of different sized proteins in the presence of deuterated lysate.
In contrast to the previous study [2], the apparent global diffusion of the different proteins investigated does significantly deviate from the total volume fraction dependence of the pure protein solutions. While small proteins have a higher diffusion coefficient in the presence of lysate compared with the pure protein solution, big proteins, however, are slowed down. These results give a new insight into the diffusive properties of proteins in cells and might contribute significantly to a quantitative understanding of biological processes.
[1] M. Grimaldo, et al., Quart. Rev. Biophys. 52 (2019) e7, 1;
[2] M. Grimaldo, et al., J. Phys. Chem. Lett. 10 (2019) 1709