SKM 2021 – scientific programme

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BP: Fachverband Biologische Physik

BP 4: Posters Biological Physics

BP 4.1: Poster

Thursday, September 30, 2021, 11:15–12:15, P

Gelation dynamics upon pressure-induced liquid-liquid phase separation in a water-lysozyme solution — Marc Moron¹, •Ahmed Al-Masoodi², Clementine Lovato², Mario Reiser³, Lisa Randolph², Göran Surmeier¹, Jennifer Bolle¹, Fabian Westermeier⁴, Michael Sprung⁴, Metin Tolan¹, Roland Winter⁵, Michael Paulus¹, and Christian Gutt² — ¹Fakultät Physik / DELTA, TU Dortmund, 44221 Dortmund, Germany — ²Department Physik, Universität Siegen, 57072 Siegen, Germany — ³Department of Physics, Stockholm University, 10691 Stockholm, Sweden — ⁴Deutsches Elektronen Synchrotron DESY, 22607 Hamburg, Germany — ⁵Fakultät Chemie und Chemische Biologie, Physikalische Chemie, TU Dortmund, 44221 Dortmund, Germany

Phase transitions in concentrated protein solutions have been in the focus of research for years. For example, many diseases can be attributed to protein aggregation or liquid-liquid phase separation in human cells. Lysozyme represents a well-studied model protein. We investigated the effect of hydrostatic pressure on concentrated lysozyme solutions in different environments and were able to show that besides temperature, protein concentration, cosolvents and ionic strength also the hydrostatic pressure modulates the protein-protein interaction. Up to now, only the static properties of the lysozyme solutions were characterized. In this work, we present first pressure dependent X-ray photon correlation spectroscopy (XPCS) measurements on concentrated lysozyme solutions to study the dynamics of pressure-induced liquid-liquid phase transitions.