SKM 2021 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 4: Posters Biological Physics
BP 4.9: Poster
Donnerstag, 30. September 2021, 11:15–12:15, P
On the adhesion-velocity relation and multistability of the motile state of MDA MB 231 cells on fibronectin lanes — Christoph Schreiber1, Behnam Amiri2, •Johannes Heyn1, Joachim Rädler1, and Martin Falcke2,3 — 1Ludwig-Maximilians-Universität München (LMU), Fakultät für Physik, Geschwister-Scholl-Platz 1, 80539 München, Germany — 2Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Robert Rössle Str. 10, 13125 Berlin, Germany — 3Dept. of Physics, Humboldt University, Newtonstr. 15, 12489 Berlin, Germany
Migration of eukaryotic cells is a fundamental process for embryonic development, wound healing, immune responses, and tumour metastasis. A universal observation is the well-known biphasic adhesion-velocity relation. There is, however, little quantitative understanding of how adhesion and intracellular forces control cell velocity. We study the motion of MDA-MB-231 cells on microlanes with fields of alternating Fibronectin densities to address this topic and derive a mathematical model from the leading-edge force balance and the force-dependent polymerization rate. It reproduces quantitatively our measured adhesion-velocity relation. All motion-related forces are controlled by adhesion and velocity, which allows motion even with higher Fibronectin density at the rear than at the front. At transitions between different Fibronectin densities, steady motion is perturbed which changes the front and rear velocity. We then discuss the role of the biphasic relation between retrograde flow velocity and friction force for transitions of motile states.