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O: Fachverband Oberflächenphysik

O 51: Poster Session IV: Poster to Mini-Symposium: Free-standing functional molecular 2D materials II

O 51.2: Poster

Dienstag, 2. März 2021, 13:30–15:30, P

From self-assembled monolayers to highly functional substrates for cryo-transmission electron microscopy (cryoTEM) — •Andreas Terfort — University of Frankfurt, Department of Chemistry, Institute of Inorganic and Analytical Chemistry, Max-von-Laue-Str. 7, 60438 Frankfurt, Germany

Major problems for the cryo-transmission electron microscopy (cryoTEM) of proteins are the stabilization of the nm-thick water film and the preservation of the particles within the field of observation without denaturation. Here we report three approaches to produce TEM grids suitable for the task.

1) Covering the supporting carbon films with a hydrophilic film to suppresses adhesion of proteins. This could be extended by introduction of carbon nanotubes, covered by the same kind of molecular film to aid charge dissipation during observation.

2) The cross linking of a polyglycerol-SAM resulted in ultrathin hydrogel membranes, which help the distribution of the unaltered proteins. Self-perforation of the membranes during the deposition process opened windows for an unaltered observation by the electron beam.

3) The modification of the hydrogel membrane with selective binding sites, by formation on-top of carbon nano-membranes, which in turn are obtained by cross-linking of aromatic monolayers by electron-beam irradiation. The bi-layer system is then modified covalent attachment of a selective binding site, which allows for the selective extraction of the analyte from e.g. protein mixtures.