Erlangen 2022 – scientific programme
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MO: Fachverband Molekülphysik
MO 8: Poster 1
MO 8.7: Poster
Tuesday, March 15, 2022, 16:30–18:30, P
Novel sample delivery system for small nanoparticles and biomolecules — •Lena Worbs1,2, Jannik Lübke1,2,3, Armando Estillore1, Amit Samanta1, and Jochen Küpper1,2,3 — 1Center for Free-ElectronLaser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Hamburg,Germany — 2Department of Physics, Universität Hamburg,Hamburg, Germany — 3Center for Ultrafast Imaging, UniversitätHamburg, Hamburg, Germany
Coherent diffractive imaging with free-electron lasers promises to allow the reconstruction of the three-dimensional molecular structures of isolated particles at atomic resolution [1]. However, because of the typically low signal-to-noise ratio, this requires the collection of a large amount of diffraction patterns. Since every intercepted particle is destroyed by the intense x-ray pulse, a new and preferably identical sample particle has to be delivered into every pulse.
We present a novel injection scheme, combining electrospray ionization for aerosolization of the sample, followed by shock-freezing and focusing techniques to produce a collimated or focused nanoparticle beams of a broad variety of biological nanoparticles, ranging from large nanoparticles to small single-domain proteins. These nanoparticle beams can be further manipulated to separate, for instance, charge states or conformational states, to allow pure samples to be delivered into the x-ray focus.
[1] M. M. Seibert, et al, Nature 470, 78 (2011).