Erlangen 2022 – scientific programme
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Q: Fachverband Quantenoptik und Photonik
Q 30: Quantum Optics (Miscellaneous) IV
Q 30.5: Talk
Wednesday, March 16, 2022, 11:45–12:00, Q-H14
Adaptive optics in Confocal microscopy and Fluorescence correlation spectroscopy — •Julius Trautmann1, Philipp Kellner1, Tiemo Anhut2, Daniel Schwedt2, and Christian Eggeling1 — 1Institut für Angewandte Optik und Biophysik, Friedrich-Schiller-Universität Jena, Philosophenweg 7, 07743 Jena — 2Carl Zeiss Microscopy GmbH, Carl-Zeiß-Promenade 10, 07745 Jena
Adaptive optics has been widely used in astronomy for the last three decades. In recent years however, it has also established itself as an important feature for high resolution microscopy. The opportunity to correct for optical aberrations is particularly useful when imaging samples with inhomogeneous refractive index structures such as cells and especially cell tissue.
Adaptive optics includes elements such as deformable mirrors (DMs) and spatial light modulators (SLMs) which can dynamically correct for aberrations.
This talk will cover the basic idea of including a deformable mirror (DM) in a confocal microscope. The optical setup will be presented and the employment of fluorescence correlation spectroscopy (FCS) will be highlighted.