Erlangen 2022 – scientific programme

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Q: Fachverband Quantenoptik und Photonik

Q 38: Photonics II

Q 38.5: Talk

Wednesday, March 16, 2022, 15:00–15:15, Q-H15

Correlative fluorescence and Soft X-ray-microscopy in the water window region in an integrated laboratory-based setup — •Sophia Kaleta¹, Julius Reinhard^{1, 2}, Felix Wiesner¹, Johann Jakob Abel¹, Martin Wünsche^{1, 2}, Jan Nathanael^{1, 2}, Katharina Reglinski³, Christian Franke³, Alexander Iliou⁴, Falk Hillmann⁴, Christian Eggeling^{3, 5}, Silvio Fuchs^{1, 2}, and Gerhard Paulus^{1, 2} — ¹IOQ, FSU Jena, Germany — ²Helmholtz Institute Jena, Germany — ³IAOB, FSU Jena, Germany — ⁴Leibniz-HKI, Jena, Germany — ⁵Leibniz-IPHT, Jena, Germany

We present a correlative fluorescence and SXR-microscope that combines both methods in an integrated setup, which allows subsequent imaging without removing the sample. While a fluorescence microscope offers functional contrast it is not sufficient for a holistic structural characterization of the sample. This gap can be closed by the correlation with other microscopy methods, for example SXR microscopy in the water window region (2.3 to 4.4nm), which allows a high natural structural contrast in biological samples. The correlation of fluorescence and SXR microscopy has already been realized at synchrotron beam sources, but not in an integrated laboratory setup as presented here. We use a laser-produced gas plasma source, based on a gas-puff target which has also been used for other X-ray and XUV imaging methods [1]. We are able to reach 100nm half pitch resolution which has been measured using a Siemens star. Additionally, we demonstrate correlative imaging of fluorescent nanobeads and cyanobacteria. [1] Skruszewicz, S., et al. Applied Physics B 127.4 (2021)