Erlangen 2022 – wissenschaftliches Programm
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Q: Fachverband Quantenoptik und Photonik
Q 64: Nano-Optics III
Q 64.4: Vortrag
Freitag, 18. März 2022, 11:15–11:30, Q-H11
On the usage of fluorescent nanodiamonds in modern nanoscopy — •Philipp Kellner1, Max Haase1, Tanja Weil3, and Christian Eggeling1,2 — 1Institut für angewandte Optik und Biophysik, Friedrich-Schiller-Universität, Philosophenweg 7, 07743 Jena — 2Leibnitz-Institut für photonische Technologien, Albert-Einstein-Straße 9, 07745 Jena — 3Max-Planck-Institut für Polymerchemie, Ackermannweg 10, 55128 Mainz
Fluorescent correlation spectroscopy (FCS) is a widely used microscopy-based, non-invasive technique for measuring mechanical and chemical properties like diffusion coefficient and concentration of specific molecules in solution, biological tissue and soft matter samples. This talk will present the basics of fluorescence correlation spectroscopy and newest insights in FCS in combination with modern Stimulated Emission Depletion (StED-) Nanoscopy using fluorescent nanodiamonds, a bright, stable, biocompatible nanoparticle as a probe. We will elaborate on the usage of the method, the nanoparticle and their combination for dynamical measurements on length-scales far below the diffraction limit. A special focus will be on the question: Are StED-FCS experiments biased by optical tweezer effects?