Erlangen 2022 – wissenschaftliches Programm
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Q: Fachverband Quantenoptik und Photonik
Q 64: Nano-Optics III
Q 64.6: Vortrag
Freitag, 18. März 2022, 11:45–12:00, Q-H11
Nanoscale Imaging of Live Cells with Confocal Interferometric Scattering (iSCAT) Microscopy — •David Albrecht1, Michelle Küppers1,3, Anna Kashkanova1, Jennifer Lühr1, and Vahid Sandoghdar1,2,3 — 1Max Planck Institute for the Science of Light, 91058 Erlangen, Germany — 2Max-Planck-Zentrum für Physik und Medizin, 91058 Erlangen, Germany — 3Friedrich-Alexander University Erlangen-Nürenberg, 91058 Erlangen, Germany
Light microscopy methods are widely used in biomedical research to investigate cellular structure and dynamics in live specimen. Label-free approaches are of particular interest to circumvent problems such as phototoxicity, functional impairment or insufficient signal that may be imposed by the label. Here, we present nanoscale imaging with confocal interferometric scattering (iSCAT) microscopy for recording label-free information from subcellular processes. iSCAT is a shot-noise limited homodyne interferometry technique, which has been extensively used for tracking nanoparticles with exquisite performance. However, application of iSCAT for cellular imaging has been hampered by a strong speckle-like background. By employing a pinhole in a confocal arrangement, we show that one can reject a large portion of the background scattering from the complex environment of a live cell. We, thus, identify cellular organelles and confirm our findings through the molecular specificity of concomitant fluorescence microscopy measurements. We also investigate the interaction of nanoscopic matter such as intracellular vesicles, lipid droplets and viruses in a cellular context at a high spatial and temporal resolution.