Regensburg 2022 – scientific programme
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BP: Fachverband Biologische Physik
BP 12: Poster 2
BP 12.20: Poster
Tuesday, September 6, 2022, 17:30–19:30, P4
Cell volume changes in confined environments on short timescales — •Felix Graf, Bob Fregin, Doreen Biedenweg, Yesaswini Komaragiri, Stefanie Spiegler, and Oliver Otto — ZIK HIKE, University of Greifswald, Greifswald, Germany
Dynamic real-time deformability cytometry (dRT-DC) is a high-throughput method for extracting the viscoelastic material properties of cells. Cells are dynamically tracked while they translocate through a microfluidic channel and deform in response to the hydrodynamic stress. We extend the time-dependent analysis of dRT-DC towards cellular volume and perform experiments on vesicles and different cell lines in a channel of 30 × 30 µ m2 cross-section with buffers as well as cell velocities resembling physiological conditions. Our measurements reveal a volume change of ≈ 5 − 10% on a millisecond timescale over the entire length of the microchannel, which is 300 µ m. We propose an explanation of our observation by water transport through transmembrane channel proteins. In preliminary experiments, we examined the relationship between the presence and amount of channel proteins, as well as the applied stress and the volume change observed in vesicles and cells. We expect our results to provide insights into the processes involved in physiological volume changes of cells in flow.