Regensburg 2022 – scientific programme

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BP: Fachverband Biologische Physik

BP 12: Poster 2

BP 12.22: Poster

Tuesday, September 6, 2022, 17:30–19:30, P4

Quantifying the relation between cell membrane and nucleus through Shape-based Voronoi tessellation — •Madhura Ramani¹, Maxime Hubert¹, Sara Kaliman¹, Simone Gehrer¹, Florian Rehfeldt², and Ana-Sunčana Smith^1,3 — ¹PULS group, FAU Erlangen-Nürnberg, Erlangen, Germany — ²Experimental Physics 1, Universität Bayreuth, Bayreuth, Germany — ³Group for Computational Life Sciences, Ruđer Bošković Institute, Zagreb, Croatia

Numerous disorders caused by genetic alterations empathize the im portance of nuclear shape and position within the cell. It is crucial to understand how the cell and nuclei relate mechanically to each other in various conditions. We investigate this relation using confluent MDCK-II monolayers grown unconstrained on substrates of various elasticities. The synergy between the cell mem brane and nucleus is measured through the quality of the Shape-based Voronoi Tessellation (SVT), which is then compared to the tessella tion of space provided by the cell membranes in the tissue. To address the precision, we compare SVT-extracted morphological information to the corresponding membrane-segmented ones and show that the method outclasses classical Voronoi Tessellation. As the SVT relies on the nuclei position to approximate the cell membrane, we present a systematic measure of the distance between the cell and nucleus center of mass. Our method offers insights regarding the mechanical feedback between the cell membrane shape and nuclei positioning, and is central in the creation of theoretical and numerical models of tissues.