Regensburg 2022 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 12: Poster 2

BP 12.23: Poster

Dienstag, 6. September 2022, 17:30–19:30, P4

Reactive oxygen species induce cell stiffening through lysosomal disruption and subsequent intracellular acidosis in HL60 cells — •Yesaswini Komaragiri^1,2, Ricardo Hugo Pires^1,2, Stefanie Spiegler^1,2, Huy Tung Dau¹, Doreen Biedenweg¹, Clara Ortegon Salas³, MD Faruq Hossain¹, Bob Fregin^1,2, Stefan Gross^2,3, Manuela Gellert³, Uwe Lendeckel³, Christopher Lillig³, and Oliver Otto^1,2 — ¹ZIK HIKE, University of Greifswald, Greifswald, Germany — ²DZHK, University Medicine Greifswald, Greifswald, Germany — ³University Medicine Greifswald, Greifswald, Germany

Reactive oxygen species (ROS) are important players of redox homeostasis and associated with cellular alterations in both, physiological and pathological conditions. Effects of different ROS on the cytoskeleton have been reported earlier; however, the exact mechanism by which they alter cell mechanics remains to be understood. Here, we used varying concentrations of hydrogen peroxide to induce intracellular ROS in human myeloid precursor cells (HL60). Using real-time fluorescence and deformability cytometry, we combined the mechanical characterization of cells with simultaneous fluorometric assessment of intracellular superoxide levels. Our work reveals a direct correlation of cell stiffening with increasing levels of superoxide. While no global changes of F-actin or microtubule networks could be observed, we show increased elastic properties as a consequence of lysosomal damage followed by intracellular acidification.