Regensburg 2022 – scientific programme

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BP: Fachverband Biologische Physik

BP 12: Poster 2

BP 12.27: Poster

Tuesday, September 6, 2022, 17:30–19:30, P4

Towards observing entry of Particulate Matter into lung cells using Photonic Force Microscopy — •Jeremias Gutekunst and Alexander Rohrbach — Lab for Bio- and Nano-Photonics, Department of Microsystems Engineering (IMTEK), University of Freiburg, Georges-Koehler-Allee 102, 79110 Freiburg, Germany,

The uptake of Particulate Matter (PM) into lung cells increases the risk of stroke and coronary heart disease. Following an in vitro approach, we expose single particulates to lung epithelial cells on a coverslip and examine their fluctuation based binding and entry paths with a photonic force microscope (PFM). The PFM consists of a highly focused laser beam, which is used to optically trap and interferometrically track a PM particle at 1 MHz frequency and with nm precision.

The central part of this work is to investigate the influence of additional scatterers below and above the nano particle of a PFM. The understanding of their optical influence is crucial, as in particle entry experiments the cell scatters light and alters the interference signal used to track the probe. We address the problem by simplifying and controlling the situation: In addition to the particle used as a probe, we introduce a further particles positioned in the same beam path, but trapped with a second laser. By decorrelating the combined scattering signals on different frequencies, we want to recover the precise position of the trapped PM probe on a broad temporal bandwidth to reliably study cell particle interactions.