Regensburg 2022 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 12: Poster 2
BP 12.31: Poster
Dienstag, 6. September 2022, 17:30–19:30, P4
Comparative investigation of F-actin using Nano IR spectroscopic and polarization resolved fluorescence microscopy imaging — •Dijo Moonnukandathil Joseph1,2, Lukas Spantzel2,3, Katharina Reglinski1,2, Asad Hafeez1,2, Yutong Wang1,2, Mohammad Soltaninezhad1,2, Christian Eggeling1,2, Rainer Heintzmann1,2, Michael Börsch2,3, and Daniela Täuber1,2 — 1Leibniz Institute of Photonic Technology, Jena — 2Friedrich-Schiller University Jena — 3University Hospital Jena, Germany
Fibrillar actin is one of the major structural components in cells. Thus, its organization has been studied extensively. Nevertheless there are still open questions, in particular, related to pathogenic infections. We examine the potential contributions of two complementary recently developed imaging methods for increasing our understanding on local F-actin: IR spectroscopic photo-induced force microscopy (PiF-IR) and 2D polarization resolved fluorescence microscopy imaging (2DPOLIM). PiF-IR provides local chemical information at high spatial resolution below 10 nm. 2DPOLIM allows to study the local aggregation of fluorescence labeled F-actin via Förster resonance energy transfer (FRET) in the range of 2-10 nm [1]. -- Funding by DAAD-GSSP, DFG-Ta1049/2 -- [1] R. Camacho et al. Commun. Biol. 2018, 1, 157.