Regensburg 2022 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 12: Poster 2
BP 12.38: Poster
Dienstag, 6. September 2022, 17:30–19:30, P4
Assessing statistical properties of resident tissue macrophages — •Miriam Schnitzerlein1,2, Anja Wegner3, Stefan Uderhardt3, and Vasily Zaburdaev1,2 — 1Department of Biology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Germany — 2Max-Planck-Zentrum für Physik und Medizin, Erlangen, Germany — 3Department of Internal Medicine 3 - Rheumatology and Immunology, Friedrich-Alexander-Universität Erlangen-Nürnberg und Universitätsklinikum Erlangen, Germany
Resident tissue macrophages (RTMs) are present in essentially all tissues in the human body. While macrophages in general are mostly known as part of the immune response, RTMs are additionally crucial for ensuring tissue homeostasis. This includes removing dead cells, providing growth factors and protecting the tissue from inflammatory damage. To monitor their surroundings, RTMs show continuous sampling behaviour by extensions and retractions of protrusions as well as endocytosis behaviour. Quantifying the growth and shrinkage of protrusions under different conditions is thereby essential to understand the overall dynamics of RTMs together with their approach of ensuring tissue homeostasis. In this project, we have employed a high-resolution intravital imaging protocol to generate movies of RTMs in vivo. Subsequently we have built an image processing pipeline to assess cell properties - such as area and perimeter of whole RTMs or the diffusion coefficient and thereby the dynamics of their protrusions. Such measurements will help to build a mathematical model for protrusion dynamics as well as to establish a biophysical model of RTMs.