Regensburg 2022 – wissenschaftliches Programm

Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe

BP: Fachverband Biologische Physik

BP 12: Poster 2

BP 12.4: Poster

Dienstag, 6. September 2022, 17:30–19:30, P4

The Weakness of Senescent Dermal Fibroblasts — •Lydia Rebehn¹, Samira Khalaji¹, Fenneke KleinJan¹, Anja Kleemann¹, Patrick Paul¹, Constantin Huster³, Ulla Nolte¹, Karmveer Singh², Taner Pula⁴, Pamela Fischer-Posovszky⁴, Karin Scharffetter-Kockanek², and Kay-E Gottschalk¹ — ¹Institute for Experimental Physics, Ulm University, Ulm, Germany — ²Department of Dermatology and Allergology, Ulm University, Ulm, Germany — ³Institut für Theoretische Physik, Universität Leipzig, Leipzig, Germany — ⁴Department of Pediatrics and Adolescent Medicine, Ulm University, Ulm, Germany

As human tissues age, there is chronological accumulation of biophysical changes from internal and environmental factors. Skin aging leads to loss of dermal matrix integrity via degradation and decreased elasticity. The mechanical properties of the dermal matrix are maintained by fibroblasts, whose properties change during replicative aging. Here, we compare biophysical properties of young versus proliferatively aged primary fibroblasts via fluorescence and traction force microscopy, single-cell AFM, and microrheology of the cytoskeleton. Results show senescent fibroblasts have decreased cytoskeletal tension and myosin II regulatory light chain phosphorylation, in addition to significant loss of traction force. The alteration of cellular forces is harmful to the process of building and maintaining extracellular matrix, while decreased cytoskeletal tension can amplify epigenetic changes involved in senescence. Exploration of these mechanical phenomena provide possibilities for unexplored pharmaceutical targets against aging.