Regensburg 2022 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 12: Poster 2
BP 12.43: Poster
Dienstag, 6. September 2022, 17:30–19:30, P4
Electrostimulation of osteoblasts on coated planar resistive electrodes — •Franziska Dorn1, Christian Völkner1, Meike Genzow2, Martina Grüning2, Sven Neuber3, Regina Lange1, Ingo Barke1, Christiane A. Helm3, Barbara Nebe2, and Sylvia Speller1 — 1Institute of Physics, University of Rostock — 2University Medical Center Rostock, University of Rostock — 3Institute of Physics, University of Greifswald
The development of electrically active implants may profit from knowledge and understanding of how osteoblasts respond to electrostimulation. Besides the aim to find routes to accelerate adhesion of osteoblasts, the cellular response in terms of migration and deformation is interesting. For our experiments we use a planar resistive electrode configuration with DC electrostimulation. The glass substrate is covered with a few bilayers of polyelectrolyte (PDADMA/PEI) with carbon nanotubes incorporated to enhance electrical conductivity. In physiologic medium the sheet resistance increases from few kΩ to more than 10 kΩ. Human osteoblast-like cells (MG-63) were seeded on the electrode and, after 24 h cell growth, stimulated by a couple of cycles at voltages between 1V and 2V. The observed cellular shape changes and mobility are only subtle and the dependence on the orientation of the electric field axes is not obvious. Experiments at an earlier phase in the adhesion process i.e., in a shorter time frame of cell adhesion, are considered. [1] C. Voelkner, et al, Beilstein J. Nanotechnol. 12, 242 (2021) [2] M. Gruening, et al, Front. Bioeng. Biotechnol. 8,1016 (2020) [3] H. Rebl, et al, Adv. Engin. Mater. 12, B356 (2010)