Regensburg 2022 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 12: Poster 2
BP 12.44: Poster
Dienstag, 6. September 2022, 17:30–19:30, P4
The zeta potential as parameter in electric field landscapes for guiding cell adhesion — •Wanda Witte1, Christian Völkner1, Regina Lange1, Susanne Seemann2, Barbara Nebe2, Ingo Barke1, and Sylvia Speller1 — 1Institute of Physics, University of Rostock, Rostock, Germany — 2Department of Cell Biology, Rostock University Medical Center, Rostock, Germany
In osseointegration of implants, chemical and physical material properties influence inital cell adhesion. The relevant surface potential for cells is the zeta potential at the shear plane or hydrodynamic distance. We investigate how the zeta potential of glass can be modified by an aggregated molecular monolayer, with the aim of using the zeta potential to create electric field landscapes. To achieve the aggregation of a molecule monolayer, amine-terminated dendrimers and albumin were deposited on the glass by micro-contact printing or immersion. An electrokinetic analyzer was used to determine the zeta potential of the coated and uncoated samples. The successful physisorption of molecules was verified by fluorescence microscopy and force microscopy (AFM). It could be shown that the application of albumin or amine-terminated dendrimers increases the zeta potential by approx. 25 eV and 40 eV. The choices and shape responses of osteoblasts (MG-65) in molecule stripe landscapes are discussed.