Regensburg 2022 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 12: Poster 2

BP 12.45: Poster

Dienstag, 6. September 2022, 17:30–19:30, P4

Quantification of the dynamics of confluent endothelial cells — •Anselm Hohlstamm, Andreas Deussen, and Peter Dieterich — Institut für Physiologie, Medizinische Fakultät, TU Dresden

Cooperative cell dynamics resulting from a complex interplay of single cell migration and cell-to-cell interactions plays a fundamental role in maintaining a confluent cell layer despite continuous changes in cell numbers and environmental conditions. It is the aim of this work to extract the essential components of this dynamics. Therefore, we seeded human umbilical vein endothelial cells and stained their nuclei with a fluorescent dye. Cells were observed within 48 hours (dt = 10 minutes). We obtained up to 50.000 cell trajectories within an area of 6 x 7 millimeters for 10 different experiments. All analyses were performed under nearly confluent conditions. Cells continued to show lively proliferations and a non-stationary behavior indicated by a two-phase decay of the mean squared velocity. This behavior is accompanied by a decay of the velocity correlation. In addition, we found an exponential repulsion between cells that could transiently rise cell velocities due to cell proliferations. We put these observations into a mathematical model coupling cell proliferation and mean squared velocities over time. Bayesian analysis was applied to determine the best model and its parameters. In summary, we are able to perform a characterization of the complex cell dynamics. This approach can be used for simulations and application to different experimental conditions.