Regensburg 2022 – scientific programme
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BP: Fachverband Biologische Physik
BP 12: Poster 2
BP 12.54: Poster
Tuesday, September 6, 2022, 17:30–19:30, P4
Single-particle Diffractive Imaging at the European XFEL: Instrumentation, Data Acquisition and Hit-finding — •Moritz Stammer1, Charlotte Neuhaus1, Jette Alfken1, Markus Osterhoff1, Richard Bean2, Johan Bielecki2, Juncheng E2, Safi Rafie-Zinedine2, Raphael de Wijn2, Romain Letrun2, Adrian Mancuso2, Reinhart Jahn3, and Tim Salditt1 — 1Georg-August-Universität, Institute for X-ray Physics, 37077 Göttingen — 2Scientific Instrument SPB/SFX, European XFEL GmbH, Holzkoppel 4, 22869 Schenefeld Germany — 3Laboratory of Neurobiology, Max Planck Institute for Multidisciplinary Sciences, 37077 Göttingen, Germany
The European XFEL provides state-of-the-art instrumentation for absolving single-pulse, single-particle coherent diffractive imaging, which we have used to investigate synaptic vesicles, harvested from rat brain, with high spatial resolution. The method involves serial bio-sample delivery by aerosol jet such that droplets incorporating single particles are probed by femto-second pulses. In this way two prevalent challenges of SAXS (polydispersity and radiation damage for high brilliance beams) can be met. Stochastic distribution of sample and a nano-focused beam means only a fraction of the recorded data was of interest (1.2 PB in total, roughly 3· 108 images). We present technical details behind the data acquisition used for this proof-of-concept experiment at the SPB instrument of the European FEL as well as our strategy in ”hit-finding”. Further, first steps towards electron density reconstruction will be presented as well as comparison to preceding SAXS work.