Regensburg 2022 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 12: Poster 2
BP 12.6: Poster
Dienstag, 6. September 2022, 17:30–19:30, P4
Neutrophil mechanotransduction during durotaxis — •Fatemeh Abbasi1, Matthias Brandt2, and Timo Betz1 — 1Third Institute of Physics-Biophysics, Georg August University Göttingen — 2Institute of Cell Biology, ZMBE, University of Münster
In Vivo, cells experience complex tissue environments with various chemical and physical features. 3D confinement is one of the major physical obstacles for cells in their natural environment. Neutrophils are among the most abundant immune cells in our body, which have to cope with various physical constrictions on their way from production to the infection site. In addition to confinement, the stiffness of the microenvironment is another mechanical feature these rapidly moving cells are exposed to. Neutrophils experience various tissue stiffness, from 1 kPa (bone marrow) to 20 MPa (bone). Previous studies have demonstrated that these cells are responsive to their microenvironment stiffness by adjusting their adhesion and spreading. Based on this knowledge we decided to combine confinement and stiffness change and investigate the impact of 3D stiffness gradient on cell behaviour and migration, a fact called durotaxis. We hypothesized that stiffness gradient might be a triggering factor of neutrophil migration toward the infection site. We confine neutrophils in between 2 layers of polyacrylamide hydrogels with 2 different stiffness and keep this distance stable for the desired period of time to investigate cell mechanotransduction during durotaxis from different points of view. Our preliminary results regarding the neutrophil durotaxis show a surprising and transient force peak on the soft substrate during cell shifting.