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BP: Fachverband Biologische Physik

BP 12: Poster 2

BP 12.62: Poster

Dienstag, 6. September 2022, 17:30–19:30, P4

Fast sorting of microfluidic droplets by content type with combined bright field and fluorescence detection — •Jonas Pfeil, Patricia Schwilling, and Othmar Marti — Universität Ulm, Ulm, Germany

Droplet-based microfluidics in context of fluorescent imaging can be used for a multitude of applications in biophysics, medicine, and lab-on-a-chip. One remaining issue in the encapsulation process of particle-like objects is that the number of encapsulated objects is Poisson or Poission-like distributed. A sorting step immediately after the encapsulation reduces the number of falsely-laden droplets.

Here we present results of sorting of beads with similar diameters and different fluorescent signals. Therefore, we encapsulate similar sized beads with different fluorescent signature and sort them using a time multiplexed imaging approach to simultaneously detect the population for each bead type. Thereby, we show that it is possible to achieve a user-defined, homogeneous configuration of fluorescent and non fluorescent particles in droplets.