Regensburg 2022 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 13: Cytoskeleton
BP 13.11: Vortrag
Mittwoch, 7. September 2022, 12:30–12:45, H15
investigating cardio-myocyte scar formation on a single cell level using ROCS microscopy — •Arash Felekary1, Alexander Rohrbach1, Stephanie Schmid2, and Eva Rog-Zielinska2 — 1IMTEK, Lab for Bio- and Nano-Photonics, Freiburg, Germany — 2Institute for Experimental Cardiovascular Medicine, Freiburg, Germany
Rotating coherent scattering (ROCS) microscopy is a label-free super-resolution microscopy technique enabling 150 nm spatial and 10 ms temporal resolution, which is highly beneficial for live-cell imaging. We have applied ROCS in total internal reflection (TIR) mode to acquire high-quality images from tunneling nanotubes (TNTs). TNTs or membrane nanotubes, are more than 10 micrometers in length and about 100 nm thin and directly connect distant cells. It seems that after heart injuries, such as myocardial infarction, mechanical and biochemical communication between heart fibroblasts (FB) and cardio myocytes (CM) is established by TNTs, which helped to generate an extracellular matrix (ECM). TNTs could be involved in the exchange of small molecules and ions between neighbor cells, injury-signal recognition, and directed collagen deposition. We measured the interaction between CMs and FBs, i.e. the dynamics of TNT fluctuations by 100 Hz ROCS movies. With a post-processing activity analysis with frequency decomposition, we detected TNT stiffening over minutes. Computer simulations of stimulated TNT motions or thermal particle motions help to confirm or reject the underlying assumptions forming a mechanistic picture.