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Regensburg 2022 – scientific programme

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BP: Fachverband Biologische Physik

BP 26: Focus Session: Bioinspired Systems

BP 26.4: Talk

Thursday, September 8, 2022, 16:15–16:30, H15

Bottom-up assembly of synthetic cells with bio-inspired DNA-based cytoskeletons — •Kevin Jahnke1, Pengfei Zhan2, Maja Illig1, Na Liu2, and Kerstin Göpfrich11Max Planck Institute for Medical Research — 2Stuttgart University

The bottom-up assembly of synthetic cells with a functional cytoskeleton sets a major milestone to understand cell mechanics and to develop man-made cellular machines. However, the combination of multiple elements and functions remained elusive, which stimulates endeavors to explore entirely synthetic bio-inspired and rationally designed solutions towards engineering life. To this end, DNA nanotechnology represents one of the most promising routes. Here, we demonstrate functional DNA-based cytoskeletons operating in microfluidic cell-sized compartments and lipid vesicles. The synthetic cytoskeletons consist of DNA tiles self-assembled into filament networks (Zhan*, Jahnke* et al., in press at Nat. Chem. 2022; Jahnke et al., ACS Nano 2022). These synthetic cytoskeletons can be rationally designed and controlled to imitate features of natural cytoskeletons, including ATP-triggered polymerization, morphology control and vesicle transport in cell-sized confinement. Also, they possess engineerable characteristics, including assembly and disassembly powered by DNA hybridization, light or aptamer-target interactions. Moreover, we incorporate membrane-spanning DNA origami signalling units to allow for mechanochemical signal transduction across the GUV membrane (Jahnke, Illig et al., biorxiv 2022). This work underpins DNA nanotechnology as a key player in building synthetic cells from the bottom up.

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