Regensburg 2022 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 28: Biopolymers, Biomaterials and Bioinspired Functional Materials (joint session CPP/BP)
BP 28.2: Vortrag
Freitag, 9. September 2022, 10:00–10:15, H39
A Semisynthetic Superparamagnetic Nanoprobe for Protein Targeting and Manipulation — •Andreas Neusch1, Iuliia Novoselova1, Nikolaos Tetos2, Michael Farle2, Ulf Wiedland2, and Cornelia Monzel1 — 1Heinrich-Heine University Düsseldorf, Germany — 2University of Duisburg-Essen, Germany
Probing and manipulating biological functions requires tools to target and modify the proteins involved in the respective process. In recent years Magnetogenetics emerged as an approach where magnetic nanoparticles (MNPs) and external magnetic fields are used to realize such manipulation (Lisse et al., Adv. Mater., 29, 1700189 (2017)). The advantages of this combination lies within the deep tissue penetration of magnetic fields and the possibility to apply stimuli on nanoscales leading to spatial redistribution, force application, or heat generation of proteins. However, a precise active perturbation requires MNPs to be monodisperse, biocompatible, tunable with regard to their magnetic properties, as well as exhibiting a modifiable molecular shell (Monzel et al., Chem. Sci. 8, 7330-7338 (2017)). Here, we synthesize a bioinspired semisynthetic MNP - Magnetoferritin (MFt) -, which fulfils these demands. MFt is based on the globular iron storage protein complex ferritin that converts iron ions to a ferrihydrite core but can be synthetically loaded with a magnetic iron oxide core (Novoselova et al., Nanomaterials, 11, 2267 (2021)). MFt was chemically, physically and magnetically characterized both in vitro and in vivo. We demonstrate how MFt can be used to target proteins on living cells as well as to spatially manipulate MFts in a single cell environment.