Regensburg 2022 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 3: Cell Mechanics 1

BP 3.8: Vortrag

Montag, 5. September 2022, 11:45–12:00, H15

Elastic modulus of lipid-loaded platelets investigated with scanning ion conductance microscopy (SICM) — •Hendrik von Eysmondt¹, Johannes Rheinlaender¹, Madhumita Chatterjee², and Tilman E. Schäffer¹ — ¹Institute of Applied Physics, Eberhard-Karls-University Tübingen, Germany — ²Department of Cardiology and Angiology, University Hospital Tübingen, Germany

Platelets are small, anucleate blood cells involved in blood hemostasis, wound healing, and immune response as well as in diseases like atherosclerosis and coronary artery disease. Both low-density lipoprotein (LDL) and its oxidized form (OxLDL) increase the prothrombotic potential of platelets. Recently, it was shown that a chemokine receptor ACKR3/CXCR7 agonist inhibits platelet activation and thrombus formation, offering a new therapeutic choice for hyperlipidemic patients. However, the impacts of LDL, OxLDL, and CXCR7-agonist on platelet morphology and mechanics have not yet been identified.

We therefore investigated the influence of LDL, OxLDL, and CXCR7-agonist on platelet morphology and mechanics using SICM. We showed that CXCR7-agonist pre-treatment reduced the initial spreading rate on collagen, the final spreading area on both collagen and fibrinogen, and the elastic modulus on fibrinogen. We also showed that OxLDL, but not LDL, significantly alters the morphology and elastic modulus of lipid-loaded platelets and that CXCR7-agonist pretreatment can reverse some of the effects of OxLDL.