Regensburg 2022 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 5: Focus Session: Super Resolution Microscopy and Dynamics of Supramolecular Complexes

BP 5.1: Hauptvortrag

Montag, 5. September 2022, 15:00–15:30, H15

The functional nano-architecture of axonal actin — •Christophe Leterrier — Aix Marseille Université, CNRS, INP UMR7051, NeuroCyto, Marseille, France

The intricate arborization and molecular identity of axons is maintained for decades, but must also continuously adapt to changes in the environment and modulate the activity of neurons. Axons fulfill these paradoxical demands thanks to a unique cytoskeletal organization that ensures the coordinated transport, anchoring and assembly of axonal components. In our lab, we use super-resolution microscopy to delineate and map the nanoscale architecture of actin-based structures within the axon: the periodic actin/spectrin submembrane scaffold, intra-axonal hotspots and trails, and presynaptic actin assemblies. We are exploring their molecular organization and functions by combining versatile labeling approaches, correlative live-cell/super-resolution/electron microscopy and quantitative analysis that allow for high-content, nanoscale interrogation of the axonal architecture.