Regensburg 2022 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 5: Focus Session: Super Resolution Microscopy and Dynamics of Supramolecular Complexes
BP 5.3: Vortrag
Montag, 5. September 2022, 15:45–16:00, H15
Cytoskeletal organization of red blood cells during malaria infections investigated with super-resolution microscopy and pair cross-correlation analysis — Pintu Patra1, Cecilia P Sanchez2, Michael Lanzer2, and •Ulrich S Schwarz1 — 1Institute for Theoretical Physics & BioQuant, Heidelberg University, Germany — 2Center of Infectious Diseases, Parasitology, University Hospital Heidelberg, Germany
Measuring the distance between molecules is key to understanding the molecular organization of biological systems. The pair cross-correlation (PCC) function computed from two-color super-resolution microscopy images provides a measure of co-localization between differently labeled molecules. Here, we theoretically compute the PCC-function between two molecules by using 2D Gaussian distribution as the effective point spread function for single molecules. By fitting this function to simulated data based on experimentally measured images, one can estimate both small and large separation distances. We apply this method to malaria-infected red blood cells and demonstrate that the knob-associated histidine-rich protein (KAHRP), which is used by the parasite to remodel the spectrin-actin network from a distance, relocalizes from the ankyrin bridges to the actin-based junctional complexes during the 48 hours course of the infection.