Regensburg 2022 – scientific programme

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BP: Fachverband Biologische Physik

BP 5: Focus Session: Super Resolution Microscopy and Dynamics of Supramolecular Complexes

BP 5.4: Talk

Monday, September 5, 2022, 16:15–16:45, H15

Superresolution microscopy for structural cell biology — •Jonas Ries — EMBL Heidelberg

Superresolution microscopy, such as single-molecule localization microscopy (SMLM), is becoming a key technique for structural cell biology, ideally complementing electron microscopy. I will discuss projects in my group in which we push SMLM towards nanometer resolution in 3D and multicolor with the aim to investigate the structure and dynamics of molecular machines in cells. I will show how these technologies allowed us to gain mechanistic insights into the machinery that drives endocytosis. Endocytosis is an essential cellular function by which cells take up molecules from the environment. We were able to reconstruct the dynamics of this process from thousands of snapshots taken in fixed cells. I will conclude with first results illustrating the potential of MINFLUX to image dynamic structural changes of protein machines in the living cell with nanometer resolution. Specifically, I will show how we resolved the precise stepping motion of the motor protein kinesin in living cells.