Regensburg 2022 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 5: Focus Session: Super Resolution Microscopy and Dynamics of Supramolecular Complexes
BP 5.6: Vortrag
Montag, 5. September 2022, 17:00–17:15, H15
Transient Optoplasmonic Detection of Single Proteins on the Nanosecond Time Scale — •Martin D. Baaske1,2, Nasrin Asgari1, Deep Punj1, and Michel Orrit1 — 1Leiden University, Leiden, Netherlands — 2Johannes Gutenberg-University, Mainz, Germany
Label-free optical detection schemes commonly rely on specific chemical interactions between receptor and target molecule in order to facilitate analyte recognition. Here I present our first steps on a novel pathway to fingerprint proteins via analysis of their motion, i.e., physical properties such as stokes radius and polarizability rather than chemical interactions. We show that via a polarization selective technique and careful optimization of a confocal microscope single gold nanorods, which are commonly used as labels, can be transformed into high-speed nanoscale sensors. We perform photothermal spectroscopy on single gold nanorods and use it as a means to probe their sensitivity to refractive index changes with respect to the experimental parameters * in turn allowing us to optimize the later on a rod-to-rod basis. This enables the detection single protein molecules traversing plasmonic near fields with previously time resolutions on the nanosecond scale.