Parts | Days | Selection | Search | Updates | Downloads | Help
BP: Fachverband Biologische Physik
BP 5: Focus Session: Super Resolution Microscopy and Dynamics of Supramolecular Complexes
BP 5.7: Talk
Monday, September 5, 2022, 17:15–17:30, H15
Modeling the assembly and invagination of clathrin lattices at the cell membrane — •Felix Frey1,2 and Ulrich S. Schwarz3 — 1Department of Bionanoscience, Delft University of Technology, Delft, the Netherlands — 2Institute of Science and Technology Austria, Klosterneuburg, Austria — 3Institute for Theoretical Physics and BioQuant-Center, Heidelberg University, Heidelberg, Germany
Biological cells constantly relay material and information across their plasma membranes. For particles with sizes between 50 and 200 nm, clathrin-mediated endocytosis (CME) is the main uptake route. In CME clathrin triskelia assemble at the cell membrane and form a clathrin lattice. After initially growing flat the lattice starts to curve before it reaches its final size [1]. However, how this flat-to-curved transition proceeds in detail is still elusive, because theoretically several pathways can be envisioned [2]. When confronted with conventional imaging data, a microscopic model for the growth of clathrin lattices indeed suggests some level of plasticity as required for bending [3]. Recently we have combined mathematical modeling with 3D superresolution microscopy to determine the dynamics of membrane invagination. We find that membrane curvature is generated cooperatively between the triskelia of the clathrin lattice [4].
[1] D. Bucher, F. Frey et al., Nat. Commun. 9, 1109 (2018). [2] F. Frey and U.S. Schwarz, Soft Matter 16, 10723 (2020). [3] F. Frey et al., New J. of Phys. 22, 073043 (2020). [4] M. Mund et al., bioRxiv, doi:10.1101/2021.10.12.463947 (2022).