Regensburg 2022 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 7: Poster 1
BP 7.23: Poster
Montag, 5. September 2022, 18:00–20:00, P1
Modelling of cell proliferation in epithelial tissue — •Kevin Höllring1, Sara Kaliman1, Lovro Nuić2, Luca Rogić2, Simone Gehrer1, Maxime Hubert1, and Ana-Sunčana Smith1,2 — 1PULS Group, FAU Erlangen-Nürnberg, Germany — 2Group for Computational Life Sciences, Ruđer Bošković Institute, Zagreb, Croatia
The extracellular microenvironment (ECM) of epithelial cells is known to mechanically govern the properties and behavior of cells and tissues like cell differentiation, size and motility. Yet its effect on the division rate of cells in tissues has not been analyzed in detail to our knowledge. In this work, we use MDCK-II cells grown on glass and 11 kPa PDMS substrates to provide evidence for a local cell density dependent division rate in an ECM stiffness dependent manner but independent of the age of the model- tissue, its internal structure or state.
We provide a theoretical model for microscopic tissue growth in a local microenvironment with well-defined average cell-density in agreement with experimental data and Dissipative Particle Dynamics (DPD) tissue simulations. We also propose an extension to the macroscopic tissue description via the Fisher-Kolmogorov equation (FK) accounting for our new findings that is able to reproduce characteristic edge behavior of tissues that has not been able to be reproduced by the FK formalism alone.
This work therefore sheds a new light on the influence of the ECM stiffness on the maturation of epithelial tissues and the important influences of different time scales for tissue growth and cell division.