Regensburg 2022 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 7: Poster 1

BP 7.37: Poster

Montag, 5. September 2022, 18:00–20:00, P1

Investigation of biofilm formation on metal surfaces — •Bernhard Kaltschmidt¹, Annika Kiel², Ehsan Asghari², Julian Cremer³, Dario Anselmetti³, Barbara Kaltschmidt², Christian Kaltschmidt², and Andreas Hütten¹ — ¹Thin Films & Physics of Nanostructures, University of Bielefeld — ²Department of Cell Biology, University of Bielefeld — ³Biophysics & Nanosciences - "Physics of Life", University of Bielefeld

Biofilms can cause major problems in many different areas, such as corrosion and contamination of medical products. The aim of this work was to investigate biofilm formation on stainless steels and on sputtered transition metals. Steel disks of the chromium steels 1.4016, 1.4301 and 1.4510 were inoculated with bacteria in LB medium for 7 and 14 days. As bacteria we used Pseudomonas aeruginosa, which we use as our in vitro model for strong biofilm formation. After 14 days the first signs of bio-corrosion were detected by scanning electron microscopy (SEM) and atomic force microscopy. In another series of investigations, glass slides were coated with the transition metals Gold, Ruthenium and Tantal by magnetron sputtering and incubated with Pseudomonas aeruginosa for 24 hours. An uncoated glass surface served as a reference. Comparative studies of biofilm growth on the reference and on the different transition metals were carried out using SEM, confocal laser scanning microscopy and the colony forming unit assay. The analysis revealed that biofilm growth on transition metals is severely hindered compared to our non coated glass slide reference.