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BP: Fachverband Biologische Physik

BP 7: Poster 1

BP 7.3: Poster

Montag, 5. September 2022, 18:00–20:00, P1

Single Particle Tracking of Molecular Motors under Different Physiological Conditions — •Adrian Lentz, Paulina Blair, Daniel Kuckla, Philipp Hagemann, and Cornelia Monzel — Experimental Medical Physics, Heinrich-Heine University, Düsseldorf, Germany

Single particle tracking (SPT) is a powerful tool to gain insights into the dynamics of molecular motors. These proteins convert the chemical energy of adenosine triphosphate (ATP) into a forward motion to transport vesicles along the microtubule network. In our study we investigate the linear motion of the kinesin variant Kif5C, which is normally found in neuronal cells. A genetically modified Kif5C with fused green fluorescent protein was monitored with millisecond resolution and analysed with an algorithm that links precisely detected particle positions to trajectories. We establish a point density based classification of trajectories into different molecular gaits and determine diffusion, velocity and processivity of the Kif5c motor. We show a systematic analysis of the influence of different acquisition times on the motor motion determination and compare Kif5C transport in the human cell lines HeLa, MCF-7, HEK, NIH/3T3 and COS-7. Of central interest was then to measure the effect of different physiological conditions, e.g. absence or high concentration of glucose and high amount of ATP, on the Kif5C dynamics.