Regensburg 2022 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 7: Poster 1
BP 7.42: Poster
Montag, 5. September 2022, 18:00–20:00, P1
Analyses of the outer membrane of vital mitochondria — •Eric Lieberwirth1, Christian Völkner1, Regina Lange1, Anja Schaeper2, Magdalena Otte2, Armin Springer3, Markus Frank3, Ingo Barke1, Simone Baltrusch2, and Sylvia Speller1 — 1University of Rostock, Institute of Physics, 18059 Rostock, Germany — 2Rostock University Medical Center, Institute for Medical Biochemistry and Molecular Biology, 18057 Rostock, Germany — 3Rostock University Medical Center, Medical Biology and Electron Microscopy Center, 18057 Rostock, Germany
A network of mitochondria enables a cell to perform oxidative metabolism. These organelles have a double membrane that is subject to constant remodeling during the regular fusion and fission processes. This study aims to gain more knowledge about the outer membrane containing translocase, porin and ion channels. Via Scanning Ion Conductance Microscopy (SICM) is it possible to measure the outer membrane of vital mitochondria at lateral spatial resolution of approx. 50 nm and at height resolution of a few nanometer. We immobilize the organelle in phosphate buffered saline (PBS) on collagen-coated substrates and scan the outer membrane with nanopipettes. Though the nanoprobe was, on each pixel, approached from top, the observed shapes exhibit forward-backward hysteresis and flat plateaus. The corrugation amplitude amounts to a few 10 nm and soft steps are present. Labeling translocase of the outer membrane (TOM) with nanoscopic gold particles may help learning about their spatial distribution and help to identify signatures in SICM and SEM.