Regensburg 2022 – scientific programme

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BP: Fachverband Biologische Physik

BP 7: Poster 1

BP 7.44: Poster

Monday, September 5, 2022, 18:00–20:00, P1

adaptive interferometric light-sheets for resolution enhanced imaging — •meelad lalenejad and alexander rohrbach — Laboratory for Bio- and Nano-Photonics, Department of Microsystems Engineering (IMTEK), University of Freiburg, Georges-Koehler-Allee 102, 79110 Freiburg, Germany

The success of light-sheet microscopy bases on the idea that only the parts of the object being are illuminated with laser beams from the side, which are in focus of the objective lens. This concept leads to increased image contrast and reduces photo-bleaching / -toxicity. In addition, larger volumes are scanned plane-wise or line-wise, such that LSM is significantly faster than point-wise scanning methods. However, compromises in spatial resolution have had to be made because of objective lenses with limited numerical aperture and aberrations from light scattering. On the detection side, such phase aberrations could often be corrected with adaptive optics. However, spatial light modulation and phase adaptation of the illumination side still leave plenty of room for improvements. In our research we want to combine the principles of holographically shaped illumination beams with interferometric arrangements of the illumination beams. Since the modulation contrast can be deteriorated by refractive index inhomogeneities of the sample, future phase adaptation for each of the counter propagating beams shall be used as an effective aberration compensation. Using the principles of structured illumination microscopy, we show 3D images in scattering media such as cancer cell clusters obtained from two laterally scanned, counter-propagating Bessel beams.