Regensburg 2022 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 7: Poster 1
BP 7.46: Poster
Montag, 5. September 2022, 18:00–20:00, P1
Novel concepts in scanned light-sheet microscopy to improve speed, contrast and resolution — •Yatish Yatish1,2,3 and Alexander Rohrbach1,2 — 1Laboratory for Bio- and Nano-Photonics, Department of Microsystems Engineering-IMTEK, University of Freiburg, 79110 Freiburg, Germany — 2CIBSS - Centre for Integrative Biological Signalling Studies, Freiburg, Germany — 3Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Freiburg, Germany
Light-sheet microscopy (LSM) enables fast 3D, high contrast imaging offering effective sectioning and low photo-toxicity. LSM allows to investigate the issue of light scattering in both the illumination and detections, and to better understand the complex image formation. Switchable computer holograms can generate special Bessel beams that are scanned through the object offering increased penetration depths due to their self-reconstruction capability. These beams generate images with better contrast and resolution, when combined with confocal line detection. A future challenge will be to automatically adapt the illumination beam dimensions to the specific structure of object to enhance the 3D image quality. We have investigated the propagation of different beams through classes of spheres. All experiments were performed in combination with advanced computer simulations to better understand the effects of scattering. This includes the loss in quality of bead images along the optical illumination and detection axes through bead clusters, but also the position dependent scattering and absorbing of illumination and fluorescence light in cancer cell clusters.