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BP: Fachverband Biologische Physik

BP 7: Poster 1

BP 7.5: Poster

Montag, 5. September 2022, 18:00–20:00, P1

Quantification of molecule-spanning protein dynamics with fluorescence correlation spectroscopy — •Veronika Frank¹, Jean-Benoît Claude², Jérôme Wenger², and Thorsten Hugel^1,3 — ¹Institute of Physical Chemistry, University of Freiburg, Germany — ²Fresnel Institute, CNRS, Aix Marseille University, France — ³Signaling research centers BIOSS and CIBSS, University of Freiburg, Germany

Protein conformational kinetics and their regulation occur on many time and length scales. Fluorescence methods have mainly focused on the hundreds of microseconds to minutes time scale and NMR on the picosecond time scale. Here we explore the several nanoseconds to microseconds time scale for the multi-domain molecular chaperone heat shock protein Hsp90, a homodimer with a molecular weight of 90 kDa per monomer. Hsp90 is a therapeutic target for cancer therapy, but its dynamics and its dynamic interactions with other proteins are not yet fully understood.

Here we use fluorescence correlation spectroscopy and zero-mode waveguide nanoapertures to measure and understand fast molecule-spanning dynamics and how they are affected by interactors.