Regensburg 2022 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 7: Poster 1
BP 7.8: Poster
Montag, 5. September 2022, 18:00–20:00, P1
Reversible protein immobilization and biosensing on liquid-gated GFETs — •Mykola Fomin1, Lara Jorde2, Changjiang You2, Jacob Piehler2, and Carola Meyer1 — 1Department of Physics, Osnabrück University, Germany — 2Department of Biology/Chemistry and CellNanOs Center, Osnabrück University, Germany
Apart from the standard requirements such as selectivity, sensitivity, and biological compatibility, effective electronic biosensors are facing the demand for the fabrication of cost-efficient devices. Cost and resource efficiency would benefit from re-useage of the biosensor after detection, which can be achieved by reversible immobilization of the molecules in question. While multiple biosensors have already been demonstrated to generate a response to a specific analyte molecule and its various concentrations, reversible protein monitoring remains a challenging task. Besides, during such measurements on graphene exists a risk of unspecific attachment to the channel with following protein denaturation. This work aims to demonstrate reversible protein immobilization detected by current changes of a liquid-gated GFET. PEG-tris-NTA on a lipid monolayer is used for site-specific immobilization of histidine-tagged proteins. The lipid layer forms a 2,5 nm hydrophilic cover over the channel that prevents unspecific protein attachment and its denaturation, resulting in enhanced biocompatibility [1]. We track the current changes upon attachment of histidine-tagged GFP to the device and their removal upon imidazole wash.
[1] L. Jorde. et al.(2021), doi: 10.1063/5.0035871.