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SKM 2023 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 1: Membranes, Vesicles, Synthetic Cells

BP 1.2: Vortrag

Montag, 27. März 2023, 09:45–10:00, BAR Schö

Surface-induced phase separation of reconstituted nascent integrin clusters — •Chiao-Peng Hsu1, Jonas Aretz2, Reinhard Fässler2, and Andreas Bausch11Center for Functional Protein Assemblies and Lehrstuhl für Zellbiophysik (E27), Physics Department, Technische Universität München, Garching, Germany — 2Max Planck Institute of Biochemistry, Martinsried, Germany

Integrin adhesion complexes are essential membrane-associated cellular compartments for multi-cellular life. While biomolecular condensates organize specific functions in cells, cell membranes can regulate the positions and dynamics of many biomolecular condensates. Yet, the role played by membrane surfaces in the formation of initial integrin adhesion complexes still needs to be fully understood. Here, we report that phosphoinositides containing lipid membranes induce minimal integrin adhesion condensates composed of integrin β tails, kindlin, talin, paxillin, and FAK at physiological ionic strengths and protein concentrations. We show that the presence of phosphoinositides is key to enriching kindlin and talin on the membrane, forming first nascent integrin complexes, which in turn are necessary to further nucleate condensates. These results demonstrate that the biophysical properties of lipid membranes are key for inducing specific membrane-associated condensates throughout the cell.

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