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BP: Fachverband Biologische Physik

BP 1: Membranes, Vesicles, Synthetic Cells

BP 1.7: Invited Talk

Monday, March 27, 2023, 11:15–11:45, BAR Schö

Cell-free expression of membrane proteins and control of their spatial organization in synthetic lipid membranes — •Jan Steinkühler — Northwestern University, Evanston, USA — Georg-August-Universität Göttingen, Germany

Cell-free expression (CFE) is a powerful tool for synthesizing proteins outside of living cells, including membrane proteins. In this talk, we will discuss the factors that affect the yield of synthesized membrane proteins in CFE systems, including ribosome stalling and the balance between peptide-membrane association and peptide aggregation rates. We will also present a quantitative kinetic model that can be used to rationalize the engineering of protein N-terminal domain sequences and membrane composition for improved membrane protein synthesis. In addition to covering the synthesis of natural membrane proteins, we will show how CFE of de novo membrane protein designs can be used to study the role of membrane-protein hydrophobic mismatch in protein integration and organization in synthetic lipid membranes. [DOI:10.1101/2022.06.01.494374]. Our findings provide insight into protein organization in biological membranes and a framework for building up of synthetic cell membranes with new functions.