SKM 2023 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 11: Poster Session I

BP 11.12: Poster

Dienstag, 28. März 2023, 12:30–15:30, P1

Homogenization of DPPC films deposited from the gas phase onto silicon substrates — •N. Moraga¹, D. Saavedra¹, N. Gomez-Vierling¹, M. Cisternas², M.J. Retamal³, and U.G. Volkmann¹ — ¹Institute of Physics and CIEN-UC, Pontificia Universidad Catolica de Chile, Santiago, Chile — ²School of Industrial Engineering, Universidad de Valparaiso, Santiago, Chile — ³Engineering Faculty, Universidad Finis Terrae, Santiago, Chile

Supported lipid bilayers (SLBs) are stable structures that allow us to gain insight into the physical behavior of cell membranes through thin film characterization techniques. In this work, DPPC SLBs are made through Physical Vapor Deposition (PVD) technique on silicon substrates without using any solvent [1]. The film thickness was monitored in situ by high-resolution ellipsometry. The DPPC deposition rate, substrate temperature during deposition and post deposition membrane annealing temperature in vacuum and in dry air are used as parameters. Homogeneity of the phospholipid bilayer is observed through the topographical analysis and Young modulus by AFM. Lower deposition rates and a slight increase of substrate temperature led to more homogeneous films. The right annealing temperature and time further improve membrane quality to favor protein insertion [2].

Acknowledgments: Fondecyt 1180939 (UGV) and ANID doctoral grants (NM and NGV)

References:

[1] M. A. Cisternas, et al., Int. J. Mol. Sci. 21 (18), (2020) 6819.

[2] Dzikovski, B.G., et al., J Phys Chem B 2011, 115(1), 176-185.