SKM 2023 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 11: Poster Session I

BP 11.23: Poster

Dienstag, 28. März 2023, 12:30–15:30, P1

Self-regulation of mRNA expression via LNP-based incoherent feed-forward loops — •Judith A. Müller and Joachim O. Rädler — Ludwig-Maximilians-Universität, Munich

Lipid Nanoparticles (LNPs) have revolutionized the delivery of nucleic acid to living cells, including messenger RNAs (mRNAs) and small non-coding RNAs. However, at the single cell level, delivery of LNPs is heterogeneous and the expression level and timing is poorly controlled. A frequently occurring motif in natural gene regulation are incoherent feedforward loops (iFFLs) consisting of simultaneous initiation of activating transcription factors and down-regulating micro-RNAs. Here we realize lipid nanoparticles containing iFFL by ratiometric codelivery of eGFP coding mRNA and eGFP targeting siRNA. We find faster and more homogenous expression in eGFP time courses using Live Imaging on Single Cell Arrays (LISCA). The steady states levels show power law decrease as a function of siRNA/RNA ratio. Our approach demonstrates self-regulated expression via iFFL-LNP based genetic programs.