SKM 2023 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 11: Poster Session I
BP 11.44: Poster
Dienstag, 28. März 2023, 12:30–15:30, P1
Bio-SAXS of Single-Stranded DNA-Binding Proteins: Radiation Protection by the Compatible Solute Ectoine — •Marc Benjamin Hahn1, Dorothea C. Hallier1,2,3, Glen J. Smales2,3, and Harald Seitz1 — 1Bundesanstalt für Materialforschung und prüfung (BAM), 12205 Berlin, Germany — 2Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses (IZI-BB), 14476 Potsdam, Germany — 3Universität Potsdam, Institut für Biochemie und Biologie, 14476 Potsdam, Germany
Small-angle X-ray scattering (SAXS) can be used for structural determination of biological macromolecules and polymers in their native states. To improve the reliability of such experiments, the reduction of radiation damage occurring from exposure to X-rays is needed.One method, is the use of scavenger molecules that protect macromolecules against radicals produced by radiation exposure.In this study we investigate the feasibility to apply the compatible solute, osmolyte and radiation protector Ectoine (THP(B)) as a scavenger throughout SAXS measurements of single-stranded DNA-binding protein Gene-V Protein (G5P/GVP). Therefore we monitor the radiation induced changes of G5P during bio-SAXS. The resulting microscopic energy-damage relation was determined by particle scattering simulations with TOPAS/Geant4. The results are interpreted in terms of radical scavenging as well as post-irradiation effects, related to preferential-exclusion from the protein surface. Thus, Ectoine provides an non-disturbing way to improve structure-determination of proteins via bio-SAXS in future studies.