SKM 2023 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 11: Poster Session I
BP 11.48: Poster
Dienstag, 28. März 2023, 12:30–15:30, P1
Diffusive anchorage of molecular motors facilitates robust cargo transport — •Rachele Catalano1, Gina A. Monzon Monzon1,2, Rahul Grover1, Ludger Santen2, and Stefan Diez1,3 — 1B CUBE - Center for Molecular Bioengineering, TU Dresden — 2Center for Biophysics, Department of Physics, Saarland University — 3Cluster of Excellence Physics of Life, TU Dresden
Intracellular transport of vesicles and organelles is carried out by teams of molecular motor proteins moving cargo along polar intracellular filaments. Multiple-motors are coupled to each other via a fluid membrane that allows motors to diffuse along the cargo surface. How the number of involved motors and the diffusivity of motors on the cargo surface influence such transport is not well understood. Here we use a combined experimental and theoretical approach to investigate the impact of motor number and motor-cargo interaction on the motility parameters of kinesin-driven cargoes moving along microtubules. We found that the velocities of cargoes with highly diffusive motors decrease with an increase in motor number. Cargoes with non-diffusively bound motors moved with velocities independent of the motor number. Numerical simulations reveal that diffusive motor-cargo binding results in higher numbers of microtubule-bound motors, which increases steric hindrance associated with cargo slow down. Additionally, the higher number of microtubule bound motors enhances cargo run length and increases transport robustness. Our results demonstrate that loose mechanical coupling of multiple motors by diffusive membrane anchorage leads to robust transport at the cost of lower velocity.