SKM 2023 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 14: Focus Session: From Inter-individual Variability to Heterogeneous Group Dynamics and Disorder in Active Matter (joint session DY/BP/CPP)

BP 14.9: Vortrag

Mittwoch, 29. März 2023, 12:30–12:45, ZEU 160

Characterization of spatial heterogeneities as influencing factors on the dynamics of confluent endothelial cell migration — •Anselm Hohlstamm, Andreas Deussen, Stephan Speier, and Peter Dieterich — Institut für Physiologie, TU Dresden

Confluent endothelial cells are in perpetual movement. Their collective dynamics arises from the interplay of self-propelled motility and various distance-related cell interactions. However, an understanding of collective cell dynamics is complicated by large spatial heterogeneities and local cluster formations. It is the aim of this work to quantify and characterize their influence on the dynamics of cell migration. We used human umbilical vein endothelial cells, which were stained with a fluorescent dye and observed for 48 hours via time-lapse microscopy. With automated image segmentation we could track several 10.000 cells. Cell densities and mean squared velocities showed a heterogeneous spatial distribution with an inverse relation to each other. Higher cell densities also affected the strength of the velocity autocorrelation, whereas correlation times remained mostly stable during experiments. However, cell division increased the mean squared velocity without changing temporal correlations. In parallel, the mean squared displacement characterized regions with short superdiffusive phases in an aging, highly non-stationary system. In addition, local dynamics are coupled by long range spatial correlations. In summary, the dynamics of an entire endothelial layer is influenced by interactions of small heterogenous regions. Next, we will use this approach to compare different endothelial cells.